Part:BBa_K1727003

Epinecidin-1 with signal peptide

Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptide that has extensive abilities to kill or inhibit the growth of bacteria. It plays a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies.To increase efficiency in isolating our AMPs, we introduced a signal peptide upstream of the N-terminal of antimicrobial peptides to facilitate peptide production.

Peptide structure prediction

We had modified the amino acid sequence by attached an Ala at the N-terminal of Signiferin to facilitate the secreting process. And we had used structure prediction software to analysis if the attached Ala affect the peptide folding process or not.

Fig.2 The result of structure prediction

Through the secondary structure predicted. The result shows the attached of Ala didn’t affect peptide-folding process. It is still α-helix structure.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 304
  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 247